HELP. Need Letter of Recommendation advice

25 year old with bachelors in 2016 and biomedical science masters in 2018. I have kept up with one professor from undergrad that was my chair for grad school, she isn’t replying to emails I have sent asking if she can write me a letter. I have 2 letters already from doctors I have worked with. If my old professor doesn’t reply, who else should I turn to for a letter?

Options:
-my old boss that I did research with in undergrad
-another doctor
-a couple grad school professors that may or may not remember me since its been 2 years